The Indian initiative, in creating mutant resources for the functional genomics in rice, has been instrumental in the development of 87,000 ethylmethanesulfonate (EMS)-induced mutants, of which 7,000 are in advanced generations. This multi Institutional network project was initiated in 2007 with funding from Department of Biotechnology (DBT), Government of India, and ran till 2013. The second phase of the project is being executed since November 2013.The mutants have been created in the background of Nagina 22, a popular drought- and heat-tolerant upland cultivar. EMSgardeN22 database hosts all the information about the well characterized 541 mutants for agro-morphological traits, DUS characters and SSR genotyping. Users can browse detailed information regarding agro-morphological traits, panicle architecture, DUS characters, SSR genotyping information, epicuticular wax content, and elemental analysis of the mutants in the Explore section. We have generated high quality genome sequence of Nagina 22 and uploaded its CDS region in the gene catalogue section. We are continuously expanding the database for more information and they will be uploaded in future.